[ effect of Basil [Ocimum basilicum] aqueous extract on growth changes, hematology and serum biochemical parameters in rainbow trout [Oncorhynchus mykiss]]

Use of herbs in aquaculture has become more common and rainbow trout is one of the fish that is widely cultured in most countries. The purpose of this study was to find the effect of Basil aqueous extract on growth, hematological and biochemical parameters in rainbow trout. The experimental treatments were formed by five different diets including: diet without Basil aqueous extract [control], diet with 30, 60, 120, 240 ppm Basil aqueous extract per food. Rainbow trout with an average weight of 12 +/- 2.6 g were fed with these diets for 48 days and at the end of experiment, growth parameters [FCR, SGR] and blood parameters were measured. Evaluation of FCR and SGR showed that there is no significant difference between the control and treatment group [30, 60, 120 and 240 ppm]. The result of hematological parameters were showed that basil aqueous extract increased RBC [1.24 +/- 0.04x10[6] mm[3]] in the 120 ppm group, and also increased WBC [22.7 +/- 3.4x10[3] mm3], Hemoglobin [6.3 +/- 0.6 g/dL] and the mean cell hemoglobin [57.1 +/- 3.8 pg], in the 240 ppm group. The result of biochemical parameters were showed that Basil aqueous extract decreased Cholesterol [163.7 +/- 20.15 mg/dL] and triglyceride [166.5 +/- 16.2 mg/dL] in the 30 ppm group. On the other hand, there was no significant difference in Glucose in the treatment group compare to the control. Seems that adding Basil aqueous extract to rainbow trout diet may improve the positive physiological characteristics

Isolation and Identification of Phathogenic andSaprophytic fungi from gill lesions in cultivated lyprinids [common carp, silver carp and grass carp]

Isolation and identification of fungi from gill esions. Gill speciemens from the common carp Cyprinus carpio, silver carp [Hypophthalmichthys molitrix], grass carp [Ctenopharingodon idella]. The samples were obtained from the five fish arms in Mazandaran province and transferred to mycology laboratory with ice conditon. The samples were inoculated in culture media [Sabouroed glucose agar, glucose pepton agar and corm meal agar-choloramphenicol. Chi-square and t-test. Thirty-nine species of fungi were isolated from the gill lesions. The most important pathogenic fungi were Branchiomyces, Saprolegnia, Fusarium, Phoma and Exophiala. The most frequency isolated fungi were Fusarium [46.4ko[3]/41] and saprolegnia [25ko[3]/41], whereas Exophiala [3.6ko[3]/41] was showen the lowest frequency. This is the first report of isolation oi Branchiomyces, Phoma and Exophila from fish in Iran. It is concluded that the above-mentioned isolated pathogenic fungi may play an important rule in fish diseases. Thus this study should be continued into the future